Inter-species differentiation of benign Theilerias by genomic fingerprinting with arbitrary primers.
Identifieur interne : 003996 ( Main/Exploration ); précédent : 003995; suivant : 003997Inter-species differentiation of benign Theilerias by genomic fingerprinting with arbitrary primers.
Auteurs : K. Chansiri [Thaïlande] ; S. Kawazu ; T. Kamio ; K. Fujisaki ; C. Panchadcharam ; Y. Watanapokasin ; W. Uthaisang ; C. Tananyutthawongese ; N. SarataphanSource :
- Veterinary parasitology [ 0304-4017 ] ; 1998.
Descripteurs français
- KwdFr :
- ADN des protozoaires (analyse), Amorces ADN (), Animaux, Asie du Sud-Est (épidémiologie), Australie (épidémiologie), Bovins, Génome de protozoaire, Japon (épidémiologie), Profilage d'ADN (), Profilage d'ADN (médecine vétérinaire), Reproductibilité des résultats, Spécificité d'espèce, Technique RAPD (médecine vétérinaire), Theileria (), Theileria (génétique), Theilériose (parasitologie), Theilériose (épidémiologie), Électrophorèse sur gel d'agar (médecine vétérinaire).
- MESH :
- analyse : ADN des protozoaires.
- génétique : Theileria.
- médecine vétérinaire : Profilage d'ADN, Technique RAPD, Électrophorèse sur gel d'agar.
- parasitologie : Theilériose.
- épidémiologie : Asie du Sud-Est, Australie, Japon, Theilériose.
- Amorces ADN, Animaux, Bovins, Génome de protozoaire, Profilage d'ADN, Reproductibilité des résultats, Spécificité d'espèce, Theileria.
- Wicri :
English descriptors
- KwdEn :
- Animals, Asia, Southeastern (epidemiology), Australia (epidemiology), Cattle, DNA Fingerprinting (methods), DNA Fingerprinting (veterinary), DNA Primers (chemistry), DNA, Protozoan (analysis), Electrophoresis, Agar Gel (veterinary), Genome, Protozoan, Japan (epidemiology), Random Amplified Polymorphic DNA Technique (veterinary), Reproducibility of Results, Species Specificity, Theileria (classification), Theileria (genetics), Theileriasis (epidemiology), Theileriasis (parasitology).
- MESH :
- chemical , analysis : DNA, Protozoan.
- chemical , chemistry : DNA Primers.
- geographic , epidemiology : Asia, Southeastern, Australia, Japan.
- classification : Theileria.
- epidemiology : Theileriasis.
- genetics : Theileria.
- methods : DNA Fingerprinting.
- parasitology : Theileriasis.
- veterinary : DNA Fingerprinting, Electrophoresis, Agar Gel, Random Amplified Polymorphic DNA Technique.
- Animals, Cattle, Genome, Protozoan, Reproducibility of Results, Species Specificity.
Abstract
A method was developed to obtain reproducible DNA fingerprints from five distinct purified benign Theileria genomic DNAs by PCR-based amplification. Randomly amplified polymorphic DNA (RAPD) profiles were obtained from 10 randomly designed 12-mers. However, nine of the 10 primers could generate the difference in RAPD-PCR profiles which allowed discrimination of Theileria species. The method has advantage of being simple, fast and sensitive for diagnosis and characterization of the parasites since it does not require prior DNA sequence information to construct species-specific probes or primers. The results are also beneficial for a proper understanding of the epidemiology and designing rational control programmes for Theileriosis in Asian and South-East Asian countries.
DOI: 10.1016/s0304-4017(98)00155-1
PubMed: 9806494
Affiliations:
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Kamio</name></author><author><name sortKey="Fujisaki, K" sort="Fujisaki, K" uniqKey="Fujisaki K" first="K" last="Fujisaki">K. Fujisaki</name></author><author><name sortKey="Panchadcharam, C" sort="Panchadcharam, C" uniqKey="Panchadcharam C" first="C" last="Panchadcharam">C. Panchadcharam</name></author><author><name sortKey="Watanapokasin, Y" sort="Watanapokasin, Y" uniqKey="Watanapokasin Y" first="Y" last="Watanapokasin">Y. Watanapokasin</name></author><author><name sortKey="Uthaisang, W" sort="Uthaisang, W" uniqKey="Uthaisang W" first="W" last="Uthaisang">W. Uthaisang</name></author><author><name sortKey="Tananyutthawongese, C" sort="Tananyutthawongese, C" uniqKey="Tananyutthawongese C" first="C" last="Tananyutthawongese">C. Tananyutthawongese</name></author><author><name sortKey="Sarataphan, N" sort="Sarataphan, N" uniqKey="Sarataphan N" first="N" last="Sarataphan">N. 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Chansiri</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand. kosum@psm.swu.ac.th</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok</wicri:regionArea><wicri:noRegion>Bangkok</wicri:noRegion></affiliation></author><author><name sortKey="Kawazu, S" sort="Kawazu, S" uniqKey="Kawazu S" first="S" last="Kawazu">S. Kawazu</name></author><author><name sortKey="Kamio, T" sort="Kamio, T" uniqKey="Kamio T" first="T" last="Kamio">T. Kamio</name></author><author><name sortKey="Fujisaki, K" sort="Fujisaki, K" uniqKey="Fujisaki K" first="K" last="Fujisaki">K. Fujisaki</name></author><author><name sortKey="Panchadcharam, C" sort="Panchadcharam, C" uniqKey="Panchadcharam C" first="C" last="Panchadcharam">C. Panchadcharam</name></author><author><name sortKey="Watanapokasin, Y" sort="Watanapokasin, Y" uniqKey="Watanapokasin Y" first="Y" last="Watanapokasin">Y. Watanapokasin</name></author><author><name sortKey="Uthaisang, W" sort="Uthaisang, W" uniqKey="Uthaisang W" first="W" last="Uthaisang">W. Uthaisang</name></author><author><name sortKey="Tananyutthawongese, C" sort="Tananyutthawongese, C" uniqKey="Tananyutthawongese C" first="C" last="Tananyutthawongese">C. Tananyutthawongese</name></author><author><name sortKey="Sarataphan, N" sort="Sarataphan, N" uniqKey="Sarataphan N" first="N" last="Sarataphan">N. Sarataphan</name></author></analytic><series><title level="j">Veterinary parasitology</title><idno type="ISSN">0304-4017</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Asia, Southeastern (epidemiology)</term><term>Australia (epidemiology)</term><term>Cattle</term><term>DNA Fingerprinting (methods)</term><term>DNA Fingerprinting (veterinary)</term><term>DNA Primers (chemistry)</term><term>DNA, Protozoan (analysis)</term><term>Electrophoresis, Agar Gel (veterinary)</term><term>Genome, Protozoan</term><term>Japan (epidemiology)</term><term>Random Amplified Polymorphic DNA Technique (veterinary)</term><term>Reproducibility of Results</term><term>Species Specificity</term><term>Theileria (classification)</term><term>Theileria (genetics)</term><term>Theileriasis (epidemiology)</term><term>Theileriasis (parasitology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des protozoaires (analyse)</term><term>Amorces ADN ()</term><term>Animaux</term><term>Asie du Sud-Est (épidémiologie)</term><term>Australie (épidémiologie)</term><term>Bovins</term><term>Génome de protozoaire</term><term>Japon (épidémiologie)</term><term>Profilage d'ADN ()</term><term>Profilage d'ADN (médecine vétérinaire)</term><term>Reproductibilité des résultats</term><term>Spécificité d'espèce</term><term>Technique RAPD (médecine vétérinaire)</term><term>Theileria ()</term><term>Theileria (génétique)</term><term>Theilériose (parasitologie)</term><term>Theilériose (épidémiologie)</term><term>Électrophorèse sur gel d'agar (médecine vétérinaire)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Protozoan</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Asia, Southeastern</term><term>Australia</term><term>Japan</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN des protozoaires</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Theileria</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Theileriasis</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Theileria</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Theileria</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>DNA Fingerprinting</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Profilage d'ADN</term><term>Technique RAPD</term><term>Électrophorèse sur gel d'agar</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Theilériose</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Theileriasis</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>DNA Fingerprinting</term><term>Electrophoresis, Agar Gel</term><term>Random Amplified Polymorphic DNA Technique</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Asie du Sud-Est</term><term>Australie</term><term>Japon</term><term>Theilériose</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cattle</term><term>Genome, Protozoan</term><term>Reproducibility of Results</term><term>Species Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Animaux</term><term>Bovins</term><term>Génome de protozoaire</term><term>Profilage d'ADN</term><term>Reproductibilité des résultats</term><term>Spécificité d'espèce</term><term>Theileria</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Australie</term><term>Japon</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A method was developed to obtain reproducible DNA fingerprints from five distinct purified benign Theileria genomic DNAs by PCR-based amplification. Randomly amplified polymorphic DNA (RAPD) profiles were obtained from 10 randomly designed 12-mers. However, nine of the 10 primers could generate the difference in RAPD-PCR profiles which allowed discrimination of Theileria species. The method has advantage of being simple, fast and sensitive for diagnosis and characterization of the parasites since it does not require prior DNA sequence information to construct species-specific probes or primers. The results are also beneficial for a proper understanding of the epidemiology and designing rational control programmes for Theileriosis in Asian and South-East Asian countries.</div></front></TEI><affiliations><list><country><li>Thaïlande</li></country></list><tree><noCountry><name sortKey="Fujisaki, K" sort="Fujisaki, K" uniqKey="Fujisaki K" first="K" last="Fujisaki">K. Fujisaki</name><name sortKey="Kamio, T" sort="Kamio, T" uniqKey="Kamio T" first="T" last="Kamio">T. Kamio</name><name sortKey="Kawazu, S" sort="Kawazu, S" uniqKey="Kawazu S" first="S" last="Kawazu">S. Kawazu</name><name sortKey="Panchadcharam, C" sort="Panchadcharam, C" uniqKey="Panchadcharam C" first="C" last="Panchadcharam">C. Panchadcharam</name><name sortKey="Sarataphan, N" sort="Sarataphan, N" uniqKey="Sarataphan N" first="N" last="Sarataphan">N. Sarataphan</name><name sortKey="Tananyutthawongese, C" sort="Tananyutthawongese, C" uniqKey="Tananyutthawongese C" first="C" last="Tananyutthawongese">C. Tananyutthawongese</name><name sortKey="Uthaisang, W" sort="Uthaisang, W" uniqKey="Uthaisang W" first="W" last="Uthaisang">W. Uthaisang</name><name sortKey="Watanapokasin, Y" sort="Watanapokasin, Y" uniqKey="Watanapokasin Y" first="Y" last="Watanapokasin">Y. Watanapokasin</name></noCountry><country name="Thaïlande"><noRegion><name sortKey="Chansiri, K" sort="Chansiri, K" uniqKey="Chansiri K" first="K" last="Chansiri">K. Chansiri</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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